Morphological and Immunohistochemical Features of Secondary Immune Organs (Regional Lymph Nodes and Spleen) in Acute Secondary Peritonitis

Khaydarov , Azizjon, Eshbaev , Erkin, Хайдаров, А, Эшбаев, Э, Xaydarov , Azizjon, Eshbaev , Erkin

Medical science of Uzbekistan / O'zbekiston tibbiyot ilmi · 2026-yil

Annotatsiya

Aim. To characterize morphological and immunohistochemical (IHC) features of mesenteric lymph nodes and spleen in fatal acute secondary peritonitis, using age-matched non-septic tissue as a qualitative reference. Materials and Methods. Autopsy-based cross-sectional study at the Republican Pathological Anatomy Center (2018–2023). Mesenteric lymph nodes and spleen were examined from 131 adults (18–45 years) who died of acute secondary peritonitis, alongside 15 age-matched non-septic controls (acute myocardial infarction, no systemic inflammation) that served as a qualitative reference. Specimens were fixed in 10% buffered formalin, embedded in paraffin, and stained with hematoxylin-eosin (H&E); 33 lymph node specimens underwent IHC for CD3, CD4, CD20 and CD68, and 32 spleen specimens for CD8, CD68 and Ki-67, with DAB detection. Results are expressed as counts, percentages with 95% confidence intervals (Wilson score method), or mean ± standard deviation (M ± SD). Results. Within 24–72 hours, both organs showed secondary lymphoid collapse: germinal-center depletion, marginal-zone denudation, T- and B-lymphocyte migration toward inflamed foci, reticulocyte and macrophage proliferation, and interstitial edema. In lymph nodes, CD68 macrophage positivity occurred in 27/33 cases (81.8%; 95% CI 65.6–91.4%); CD3/CD4 T-lymphocytes were low/absent in 21/33 (63.6%) and 12/33 (36.4%) cases; CD20 B-lymphocytes were reduced/absent in 32/33 (97.0%). In spleen, CD8 T-lymphocyte depletion was moderately positive in 27/32 cases (84.4%; 95% CI 68.2–93.1%); Ki-67 proliferation index was 16.21 ± 1.06%, indicating insufficient regeneration. Conclusion. Acute secondary peritonitis produces time-dependent structural and immunophenotypic collapse in lymph nodes and spleen, with T- and B-lymphocyte depletion and compensatory macrophage hyperplasia.

Maqola ma’lumotlari
MualliflarKhaydarov , Azizjon, Eshbaev , Erkin, Хайдаров, А, Эшбаев, Э, Xaydarov , Azizjon, Eshbaev , Erkin
JurnalMedical science of Uzbekistan / O'zbekiston tibbiyot ilmi
NashriyotАндижон давлат тиббиёт институти
ISSN2181-3612
Nashr sanasi2026-06-29
Jild5
Son3
Betlar20-28
TilIngliz
Ilmiy sohaTibbiyot
DOI10.56121/msu-2026-3-00015
Indeksga qo‘shilgan2026-09-26

DOI: 10.56121/msu-2026-3-00015 · Maqolaning asl sahifasi

Kalit so‘zlar

acute secondary peritonitis, mesenteric lymph node, spleen, immunohistochemistry, CD3, CD4, CD8, CD20, CD68, Ki-67, secondary immunodeficiency, острый вторичный перитонит, мезентериальный лимфатический узел, селезёнка, иммуногистохимия, CD3, CD4, CD8, CD20, CD68, Ki-67, вторичный иммунодефицит, o‘tkir ikkilamchi peritonit, mezenterial limfa tuguni, taloq, immunogistokimyo, CD3, CD4, CD8, CD20, CD68, Ki-67, ikkilamchi immunitet tanqisligi

Ilmiy soha

Tibbiyot

Iqtibos keltirish

APAKhaydarov , Azizjon, Eshbaev , Erkin, Хайдаров, А, Эшбаев, Э, Xaydarov , Azizjon, & Eshbaev , Erkin (2026). Morphological and Immunohistochemical Features of Secondary Immune Organs (Regional Lymph Nodes and Spleen) in Acute Secondary Peritonitis. Medical science of Uzbekistan / O'zbekiston tibbiyot ilmi, 5(3), 20-28. https://doi.org/10.56121/msu-2026-3-00015
GOST (OAK)Khaydarov , Azizjon va b. Morphological and Immunohistochemical Features of Secondary Immune Organs (Regional Lymph Nodes and Spleen) in Acute Secondary Peritonitis // Medical science of Uzbekistan / O'zbekiston tibbiyot ilmi. – 2026. – T. 5, № 3. – P. 20-28. – DOI: 10.56121/msu-2026-3-00015.
MLAKhaydarov , Azizjon, Eshbaev , Erkin, Хайдаров, А, Эшбаев, Э, Xaydarov , Azizjon, and Eshbaev , Erkin. “Morphological and Immunohistochemical Features of Secondary Immune Organs (Regional Lymph Nodes and Spleen) in Acute Secondary Peritonitis.” Medical science of Uzbekistan / O'zbekiston tibbiyot ilmi, 5(3), 20-28, 2026. https://doi.org/10.56121/msu-2026-3-00015
ChicagoKhaydarov , Azizjon, Eshbaev , Erkin, Хайдаров, А, Эшбаев, Э, Xaydarov , Azizjon, and Eshbaev , Erkin. “Morphological and Immunohistochemical Features of Secondary Immune Organs (Regional Lymph Nodes and Spleen) in Acute Secondary Peritonitis.” Medical science of Uzbekistan / O'zbekiston tibbiyot ilmi, 5(3), 20-28 (2026). https://doi.org/10.56121/msu-2026-3-00015
HarvardKhaydarov , Azizjon, Eshbaev , Erkin, Хайдаров, А, Эшбаев, Э, Xaydarov , Azizjon, and Eshbaev , Erkin (2026) ‘Morphological and Immunohistochemical Features of Secondary Immune Organs (Regional Lymph Nodes and Spleen) in Acute Secondary Peritonitis’, Medical science of Uzbekistan / O'zbekiston tibbiyot ilmi, 5(3), 20-28. https://doi.org/10.56121/msu-2026-3-00015
BibTeX
@article{khaydarovazizjon2026,
  title = {Morphological and Immunohistochemical Features of Secondary Immune Organs (Regional Lymph Nodes and Spleen) in Acute Secondary Peritonitis},
  author = {Khaydarov , Azizjon and Eshbaev , Erkin and Хайдаров, А and Эшбаев, Э and Xaydarov , Azizjon and Eshbaev , Erkin},
  journal = {Medical science of Uzbekistan / O'zbekiston tibbiyot ilmi},
  year = {2026},
  volume = {5},
  number = {3},
  pages = {20-28},
  doi = {10.56121/msu-2026-3-00015},
  url = {https://doi.org/10.56121/msu-2026-3-00015}
}

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