Determination of stability characteristics of pseudomonas aeruginosa strains used in an external quality assessment panel

SEYFULLAEVA Bagdagul Skenderbekovna, ABDUXALILOVA Gulnora Kudratullaevna

Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali · 2026-yil

Annotatsiya

To study the stability characteristics of Pseudomonas aeruginosa for the development of an external quality assessment (EQA) panel. Materials and Methods: The study was conducted during 2023–2025 in the reference laboratory of the Antimicrobial Resistance Center of the RSSPMCEMIPD. A total of 29 P. aeruginosa strains and one control strain — Pseudomonas aeruginosa NCTC 12934 — were included in the study. Identification was performed using conventional methods and the BD Phoenix (USA) automated system. Antimicrobial susceptibility testing and determination of minimum inhibitory concentrations (MICs) were carried out.To assess strain stability, two preservation methods were evaluated: storage at ultra-low temperature (–80 °C) and lyophilization. Under –80 °C storage conditions, testing was performed four times, and after lyophilization, three times.Results: Using the disk diffusion method, the coefficient of variation (CV) did not exceed 10% for AK30, CN10, CIP5, and LEV5. For IMI10 and MRP10, slight fluctuations in mean inhibition zone diameters were observed at 12 months; however, CV values remained within acceptable limits. For FEP30, CV reached 13–14%, but the mean values and 95% confidence intervals remained comparable across storage periods.Analysis of MIC measurements demonstrated stability of minimum inhibitory concentrations for AK30, CN10, LEV5, FEP30, and CAZ10 throughout all storage durations.Conclusion: The study of P. aeruginosa strain stability showed that not all strains retain their characteristics during storage. According to our results, 6 strains of P. aeruginosa (21%) maintained their properties and were selected for the formation of the EQA panel.

Maqola ma’lumotlari
MualliflarSEYFULLAEVA Bagdagul Skenderbekovna, ABDUXALILOVA Gulnora Kudratullaevna
JurnalJournal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali
NashriyotТошкент давлат стоматалогия институти, , «Expert Lawyers» MЧЖ
ISSN2181-9300
Nashr sanasi2026-05-06
Jild11
Son2
TilIngliz
Ilmiy sohaTibbiyot
Indeksga qo‘shilgan2026-08-27

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Kalit so‘zlar

Контроль качества, Внешняя оценка качества (ВОК), Панель, Методы хранения, Лиофилизация, Quality Control (QC), External Quality Assessment (EQA), Panel, Storage Methods, Lyophilization, Sifat nazorati, sifatni tashqi baholash, panel, saqlash usullari, liofilizatsiya

Ilmiy soha

Tibbiyot

Iqtibos keltirish

APASEYFULLAEVA Bagdagul Skenderbekovna, & ABDUXALILOVA Gulnora Kudratullaevna (2026). DETERMINATION OF STABILITY CHARACTERISTICS OF PSEUDOMONAS AERUGINOSA STRAINS USED IN AN EXTERNAL QUALITY ASSESSMENT PANEL. Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali, 11(2). https://tadqiqot.uz/index.php/biomedicine/article/view/13704
GOST (OAK)SEYFULLAEVA Bagdagul Skenderbekovna, ABDUXALILOVA Gulnora Kudratullaevna DETERMINATION OF STABILITY CHARACTERISTICS OF PSEUDOMONAS AERUGINOSA STRAINS USED IN AN EXTERNAL QUALITY ASSESSMENT PANEL // Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali. – 2026. – T. 11, № 2.
MLASEYFULLAEVA Bagdagul Skenderbekovna, and ABDUXALILOVA Gulnora Kudratullaevna. “DETERMINATION OF STABILITY CHARACTERISTICS OF PSEUDOMONAS AERUGINOSA STRAINS USED IN AN EXTERNAL QUALITY ASSESSMENT PANEL.” Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali, 11(2), 2026. https://tadqiqot.uz/index.php/biomedicine/article/view/13704
ChicagoSEYFULLAEVA Bagdagul Skenderbekovna, and ABDUXALILOVA Gulnora Kudratullaevna. “DETERMINATION OF STABILITY CHARACTERISTICS OF PSEUDOMONAS AERUGINOSA STRAINS USED IN AN EXTERNAL QUALITY ASSESSMENT PANEL.” Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali, 11(2) (2026). https://tadqiqot.uz/index.php/biomedicine/article/view/13704
HarvardSEYFULLAEVA Bagdagul Skenderbekovna, and ABDUXALILOVA Gulnora Kudratullaevna (2026) ‘DETERMINATION OF STABILITY CHARACTERISTICS OF PSEUDOMONAS AERUGINOSA STRAINS USED IN AN EXTERNAL QUALITY ASSESSMENT PANEL’, Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali, 11(2). https://tadqiqot.uz/index.php/biomedicine/article/view/13704
BibTeX
@article{seyfullaevabagdagulskenderbekovna2026,
  title = {DETERMINATION OF STABILITY CHARACTERISTICS OF PSEUDOMONAS AERUGINOSA STRAINS USED IN AN EXTERNAL QUALITY ASSESSMENT PANEL},
  author = {SEYFULLAEVA Bagdagul Skenderbekovna and ABDUXALILOVA Gulnora Kudratullaevna},
  journal = {Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali},
  year = {2026},
  volume = {11},
  number = {2},
  url = {https://tadqiqot.uz/index.php/biomedicine/article/view/13704}
}

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