In this study we aimed to obtain a novel recombinant β-galactosidase baculovirus using the Bombyx mori baculovirus – insect cell expression system. A new recombinant plasmid was constructed on the basis of the pBacPAK8 transfer vector and cloned in E. coli cells. Subsequently, a recombinant baculovirus was generated in Bombyx mori cells, and efficient expression of the β-galactosidase gene was observed. The enzymatic activity of the protein was confirmed by the ONPG assay. The results demonstrated that the baculovirus – insect cell system expands the possibilities for producing proteins of industrial significance.
| Mualliflar | Shuhrat Sh. KHASANOV, Jumanazar Ch MENGTURAYEV, Jaloliddin Abdurakhmanov, Dilmurodjon B. TURDALIYEV, Shakhnoza S. Azimova |
|---|---|
| Jurnal | Kimyo va kimyo texnologiyasi |
| Nashr sanasi | 2025-12-01 |
| Jild | 2025 |
| Son | 4 |
| Til | en |
| DOI | 10.70189/1992-9498.1691 |
DOI: 10.70189/1992-9498.1691 · Maqolaning asl sahifasi · PDF
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