In this study we aimed to obtain a novel recombinant β-galactosidase baculovirus using the Bombyx mori baculovirus – insect cell expression system. A new recombinant plasmid was constructed on the basis of the pBacPAK8 transfer vector and cloned in E. coli cells. Subsequently, a recombinant baculovirus was generated in Bombyx mori cells, and efficient expression of the β-galactosidase gene was observed. The enzymatic activity of the protein was confirmed by the ONPG assay. The results demonstrated that the baculovirus – insect cell system expands the possibilities for producing proteins of industrial significance.
| Mualliflar | Shuhrat Sh. KHASANOV, Jumanazar Ch MENGTURAYEV, Jaloliddin Abdurakhmanov, Dilmurodjon B. TURDALIYEV, Shakhnoza S. Azimova |
|---|---|
| Jurnal | Kimyo va kimyo texnologiyasi |
| Nashriyot | Тошкент кимё-технология институти |
| ISSN | 1992-9498, 2181-1121 |
| Nashr sanasi | 2025-12-01 |
| Jild | 2025 |
| Son | 4 |
| Til | Ingliz |
| DOI | 10.70189/1992-9498.1691 |
| Indeksga qo‘shilgan | 2026-09-02 |
DOI: 10.70189/1992-9498.1691 · Maqolaning asl sahifasi · PDF
| APA | Shuhrat Sh. KHASANOV, Jumanazar Ch MENGTURAYEV, Jaloliddin Abdurakhmanov, Dilmurodjon B. TURDALIYEV, & Shakhnoza S. Azimova (2025). PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS. Kimyo va kimyo texnologiyasi, 2025(4). https://doi.org/10.70189/1992-9498.1691 |
|---|---|
| GOST (OAK) | Shuhrat Sh. KHASANOV va b. PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS // Kimyo va kimyo texnologiyasi. – 2025. – T. 2025, № 4. – DOI: 10.70189/1992-9498.1691. |
| MLA | Shuhrat Sh. KHASANOV, Jumanazar Ch MENGTURAYEV, Jaloliddin Abdurakhmanov, Dilmurodjon B. TURDALIYEV, and Shakhnoza S. Azimova. “PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS.” Kimyo va kimyo texnologiyasi, 2025(4), 2025. https://doi.org/10.70189/1992-9498.1691 |
| Chicago | Shuhrat Sh. KHASANOV, Jumanazar Ch MENGTURAYEV, Jaloliddin Abdurakhmanov, Dilmurodjon B. TURDALIYEV, and Shakhnoza S. Azimova. “PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS.” Kimyo va kimyo texnologiyasi, 2025(4) (2025). https://doi.org/10.70189/1992-9498.1691 |
| Harvard | Shuhrat Sh. KHASANOV, Jumanazar Ch MENGTURAYEV, Jaloliddin Abdurakhmanov, Dilmurodjon B. TURDALIYEV, and Shakhnoza S. Azimova (2025) ‘PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS’, Kimyo va kimyo texnologiyasi, 2025(4). https://doi.org/10.70189/1992-9498.1691 |
| BibTeX | @article{shuhratshkhasanov2025,
title = {PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS},
author = {Shuhrat Sh. KHASANOV and Jumanazar Ch MENGTURAYEV and Jaloliddin Abdurakhmanov and Dilmurodjon B. TURDALIYEV and Shakhnoza S. Azimova},
journal = {Kimyo va kimyo texnologiyasi},
year = {2025},
volume = {2025},
number = {4},
doi = {10.70189/1992-9498.1691},
url = {https://doi.org/10.70189/1992-9498.1691}
} |
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