The effect of the created tissue-engineered construction based on the polyfunctional bioactive film with antimicrobial properties "Nanocel" with the addition of fibroblasts on the course of the wound process of a deep soft tissue defect was evaluated. The material for the study was 12 sexually mature male rats, in which a deep defect of soft tissues was formed on the back with resection of a section of muscle 1x1 cm in size. Observation was carried out for 1, 3, 7, 14 and 21, 28 days. During the experiments, the local status, general condition, food and water consumption, changes in body weight (every 3 days), behavioral features, intensity and nature of motor activity, coordination of movements, response to external stimuli, frequency and depth of respiratory movements, state of wool and the skin, the color of the mucous membranes, the position of the tail, the amount and type of fecal matter, clinical signs of intoxication were recorded. After decapitation, a histomorphological examination of the internal organs and the place of the formed defect was carried out. Pathomorphological studies of the internal organs of animals were carried out according to the generally accepted method. Pieces of tissue were placed in a 10% formalin solution, embedded in paraffin blocks. Sections 4-5 mm thick were stained with hematoxylin-eosin. Microscopic examination was carried out using a MIKMED-2 light microscope with a built-in camera with a magnification of 40, 100, 200, and 400 times. The results of the studied hematological and biochemical parameters of experimental animals exposed to the proposed tissue-engineered construct are within physiological norms and do not differ from the control values. When conducting a morphological study of a deep soft tissue defect using a tissue-engineered construction of a polyfunctional bioactive film with antimicrobial properties "Nanocel" with the addition of fibroblasts, it was determined that it is completely resorbed within 28 days and does not form a fibrous capsule. Based on the data of the study of histological sections of soft tissue samples, it was shown that the tissue-engineered structure creates conditions for tissue regeneration, which ensures the closure of the defect with connective tissue by adding alloofibroblasts to the composition. According to the criteria for the parameters of acute and subchronic toxicity, a tissue-engineered structure to optimize the regeneration of deep soft tissue defects has no contraindications for clinical trials and medical use. It is recommended to use the proposed tissue-engineering structure as a regulator of the wound process for better engraftment of free skin grafts in the treatment of cicatricial deformities and facial skin defects of various origins and as a preparation of the surgical field in planned-operated patients.
| Mualliflar | Khramova , Natalya, Khegay, Lyubov, Makhmudov, Alisher, Храмова, Наталья, Хегай, Любовь, Махмудов , Алишер, Xramova , Natalya, Xegay, Lyubov, Maxmudov, Alisher |
|---|---|
| Jurnal | Жамият ва инновациялар / Общество и инновации / Society and innovations |
| Nashr sanasi | 2021-07-15 |
| Jild | 2 |
| Son | 6/S |
| Betlar | 166-173 |
| Til | Rus |
| DOI | 10.47689/2181-1415-vol2-iss6/s-pp166-173 |
DOI: 10.47689/2181-1415-vol2-iss6/s-pp166-173 · Maqolaning asl sahifasi
регенеративная медицина, тканеинженерная конструкция, фибробласты, наноцел, заживление раны, дефект мягких тканей, regenerative medicine, tissue engineering construction, fibroblasts, nanocell, wound healing, soft tissue defect, regenerativ tibbiyot, to'qimalarning muhandislik tuzilishi, fibroblastlar, nanotsellalar, yaralarni davolash, yumshoq to'qimalarning defekti
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Жамият ва инновациялар / Общество и инновации / Society and innovations — barcha maqolalar