Study of the mechanism of antitumor effect of K-26-V

Enikeeva Zulfiya Makhmudovna, SALIHOV Faizullo Sayfullaevich, KAMYSHOV Sergey Viktorovich

Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali · 2023-yil

Annotatsiya

Objective: to study fragments of the mechanism of action of K-26-v: alkylating ability, influence on topoisomerase II, mitotic activity, influence on drug resistance, on p53, on CFUs. Methods: The study of mitotic activity was performed on Sara's tumor. The effect on DNA and RNA synthesis of the drug was studied using the spectrophotometric method on sarcoma 180 tumor cells. The effect of the drug on multidrug resistance and on the activity of the p53 gene on sarcoma 180 cells was studied by the level of cDNA using RT-PCR. The study of CFUs was carried out on outbred mice on the 9th day after irradiation on the “THERATRON” device at a dose of 6 Gr. Results: K-26-v inhibits DNA/RNA synthesis by 95-85% in relation to the control, etoposide, respectively, by 35-55%. The inhibitory topoisomerase activity of K-26-v was 90%, and that of etoposide was 60%. Expression of the drug resistance gene of the MDR2 gene was quantified by RT- PCR: K-26-v suppresses MDR2 expression by 91%, and etoposide by 62%. K-26-v significantly increases the expression of the p53 gene - up to 88%, and etoposide - up to 55%, which determines the greater ability of K-26-b to induce tumor apoptosis. The ability of K-26-v to cause the induction of CFUs up to 12 units was shown. Conclusions. The revealed ability of K-26-v to suppress the synthesis of NA, the activity of topoisomerases, to increase the expression of p53, and also to suppress the expression of the drug resistance gene MDR2, explains its high antitumor activity. Of particular interest is the suppression of MDR2 found in K-26-v. Stimulation of CFUs with K-26-v ensures the formation of hematopoietic and immune cells, which can protect the body from the cytotoxic effect of the therapy.

Maqola ma’lumotlari
MualliflarEnikeeva Zulfiya Makhmudovna, SALIHOV Faizullo Sayfullaevich, KAMYSHOV Sergey Viktorovich
JurnalJournal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali
NashriyotТошкент давлат стоматалогия институти, , «Expert Lawyers» MЧЖ
ISSN2181-9300
Nashr sanasi2023-05-27
Jild8
Son2
TilIngliz
Ilmiy sohaTibbiyot
Indeksga qo‘shilgan2026-08-27

Maqolaning asl sahifasi

Kalit so‘zlar

MDR2, активность топоизомеразы II, КОЕс, лекарственная устойчивость, синтез нуклеиновых кислот, CFUs, drug resistance, MDR2, nucleic acid synthesis, topoisomerase II activity, CFUlar, dorilarga chidamlilik, MDR2, nuklein kislota sintezi, topoizomeraz II faolligi

Ilmiy soha

Tibbiyot

Iqtibos keltirish

APAEnikeeva Zulfiya Makhmudovna, SALIHOV Faizullo Sayfullaevich, & KAMYSHOV Sergey Viktorovich (2023). STUDY OF THE MECHANISM OF ANTITUMOR EFFECT OF K-26-V. Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali, 8(2). https://tadqiqot.uz/index.php/biomedicine/article/view/7267
GOST (OAK)Enikeeva Zulfiya Makhmudovna, SALIHOV Faizullo Sayfullaevich, KAMYSHOV Sergey Viktorovich STUDY OF THE MECHANISM OF ANTITUMOR EFFECT OF K-26-V // Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali. – 2023. – T. 8, № 2.
MLAEnikeeva Zulfiya Makhmudovna, SALIHOV Faizullo Sayfullaevich, and KAMYSHOV Sergey Viktorovich. “STUDY OF THE MECHANISM OF ANTITUMOR EFFECT OF K-26-V.” Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali, 8(2), 2023. https://tadqiqot.uz/index.php/biomedicine/article/view/7267
ChicagoEnikeeva Zulfiya Makhmudovna, SALIHOV Faizullo Sayfullaevich, and KAMYSHOV Sergey Viktorovich. “STUDY OF THE MECHANISM OF ANTITUMOR EFFECT OF K-26-V.” Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali, 8(2) (2023). https://tadqiqot.uz/index.php/biomedicine/article/view/7267
HarvardEnikeeva Zulfiya Makhmudovna, SALIHOV Faizullo Sayfullaevich, and KAMYSHOV Sergey Viktorovich (2023) ‘STUDY OF THE MECHANISM OF ANTITUMOR EFFECT OF K-26-V’, Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali, 8(2). https://tadqiqot.uz/index.php/biomedicine/article/view/7267
BibTeX
@article{enikeevazulfiyamakhmudovna2023,
  title = {STUDY OF THE MECHANISM OF ANTITUMOR EFFECT OF K-26-V},
  author = {Enikeeva Zulfiya Makhmudovna and SALIHOV Faizullo Sayfullaevich and KAMYSHOV Sergey Viktorovich},
  journal = {Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali},
  year = {2023},
  volume = {8},
  number = {2},
  url = {https://tadqiqot.uz/index.php/biomedicine/article/view/7267}
}

Boshqa maqola uchun iqtibos tayyorlash

Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali jurnalidan boshqa maqolalar

Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali — barcha maqolalar · 2023-yil arxivi

Enikeeva Zulfiya Makhmudovna — boshqa maqolalari

Tibbiyot sohasidagi boshqa maqolalar

Tibbiyot sohasidagi OAK jurnallari