CRISPR/CAS9-MEDIATED genome editing in rice (oryza sativa l.): prospects for combined drought and salinity tolerance

Ismatova, Shahzodabonu Rizomat qizi, Turayev, Ozod Sunnatalievich, Ziyaev, Zafarjon Mashrapovich

Қўқон ДПИ. Илмий хабарлар · 2026-yil

Annotatsiya

Rice (Oryza sativa L.) is highly vulnerable to drought and salinity, two major abiotic stresses that increasingly constrain productivity in water-limited and salt- affected environments. CRISPR/Cas genome editing offers a precise strategy to modify stress-responsive genes and generate novel alleles directly in elite genetic backgrounds. This review examines the molecular and practical basis for improving rice adaptation to drought and salinity through targeted genome editing, with particular emphasis on OsRR22, DST/OsDST, and DEEPER ROOTING 1 (DRO1). Loss-of-function editing of OsRR22 has been experimentally shown to enhance salinity tolerance in rice, while CRISPR/Cas9- mediated modification of DST has generated Cas9-free mutant lines with improved water retention and increased tolerance to osmotic and salt stress. DRO1, although not primarily established as a CRISPR-derived tolerance locus, is a well-validated regulator of root growth angle and represents an important target for engineering drought avoidance through deeper root systems. The review also discusses guide-RNA design, Agrobacterium-mediated transformation, tissue-culture constraints, off-target assessment, and strategies for producing transgene-free edited plants. Current evidence indicates that combining edits affecting stomatal regulation, ion homeostasis, and root architecture could support the development of rice genotypes with broader adaptation to simultaneous or sequential drought and salinity. However, multi-environment validation, yield testing, genotype-specific transformation optimization, and careful regulatory assessment remain essential before deploying such edited materials in breeding programs.

Maqola ma’lumotlari
MualliflarIsmatova, Shahzodabonu Rizomat qizi, Turayev, Ozod Sunnatalievich, Ziyaev, Zafarjon Mashrapovich
JurnalҚўқон ДПИ. Илмий хабарлар
NashriyotҚўқон давлат педагогика институти
ISSN3030-3958
Nashr sanasi2026-10-10
Jild8
Son9
Betlar1214-1220
TilIngliz
Ilmiy sohaPsixologiya
DOI10.70728/a.series.tab.v08.i09.159
Indeksga qo‘shilgan2026-10-11

DOI: 10.70728/a.series.tab.v08.i09.159 · Maqolaning asl sahifasi

Kalit so‘zlar

Oryza sativa L., CRISPR/Cas9, genome editing, drought tolerance, salinity tolerance, OsRR22, DST, DRO1, transgene-free plants, abiotic stress

Ilmiy soha

Psixologiya

Iqtibos keltirish

APAIsmatova, Shahzodabonu Rizomat qizi, Turayev, Ozod Sunnatalievich, & Ziyaev, Zafarjon Mashrapovich (2026). CRISPR/CAS9-MEDIATED GENOME EDITING IN RICE (ORYZA SATIVA L.): PROSPECTS FOR COMBINED DROUGHT AND SALINITY TOLERANCE. Қўқон ДПИ. Илмий хабарлар, 8(9), 1214-1220. https://doi.org/10.70728/a.series.tab.v08.i09.159
GOST (OAK)Ismatova, Shahzodabonu Rizomat qizi, Turayev, Ozod Sunnatalievich, Ziyaev, Zafarjon Mashrapovich CRISPR/CAS9-MEDIATED GENOME EDITING IN RICE (ORYZA SATIVA L.): PROSPECTS FOR COMBINED DROUGHT AND SALINITY TOLERANCE // Қўқон ДПИ. Илмий хабарлар. – 2026. – T. 8, № 9. – P. 1214-1220. – DOI: 10.70728/a.series.tab.v08.i09.159.
MLAIsmatova, Shahzodabonu Rizomat qizi, Turayev, Ozod Sunnatalievich, and Ziyaev, Zafarjon Mashrapovich. “CRISPR/CAS9-MEDIATED GENOME EDITING IN RICE (ORYZA SATIVA L.): PROSPECTS FOR COMBINED DROUGHT AND SALINITY TOLERANCE.” Қўқон ДПИ. Илмий хабарлар, 8(9), 1214-1220, 2026. https://doi.org/10.70728/a.series.tab.v08.i09.159
ChicagoIsmatova, Shahzodabonu Rizomat qizi, Turayev, Ozod Sunnatalievich, and Ziyaev, Zafarjon Mashrapovich. “CRISPR/CAS9-MEDIATED GENOME EDITING IN RICE (ORYZA SATIVA L.): PROSPECTS FOR COMBINED DROUGHT AND SALINITY TOLERANCE.” Қўқон ДПИ. Илмий хабарлар, 8(9), 1214-1220 (2026). https://doi.org/10.70728/a.series.tab.v08.i09.159
HarvardIsmatova, Shahzodabonu Rizomat qizi, Turayev, Ozod Sunnatalievich, and Ziyaev, Zafarjon Mashrapovich (2026) ‘CRISPR/CAS9-MEDIATED GENOME EDITING IN RICE (ORYZA SATIVA L.): PROSPECTS FOR COMBINED DROUGHT AND SALINITY TOLERANCE’, Қўқон ДПИ. Илмий хабарлар, 8(9), 1214-1220. https://doi.org/10.70728/a.series.tab.v08.i09.159
BibTeX
@article{ismatovashahzodabonurizomatqizi2026,
  title = {CRISPR/CAS9-MEDIATED GENOME EDITING IN RICE (ORYZA SATIVA L.): PROSPECTS FOR COMBINED DROUGHT AND SALINITY TOLERANCE},
  author = {Ismatova, Shahzodabonu Rizomat qizi and Turayev, Ozod Sunnatalievich and Ziyaev, Zafarjon Mashrapovich},
  journal = {Қўқон ДПИ. Илмий хабарлар},
  year = {2026},
  volume = {8},
  number = {9},
  pages = {1214-1220},
  doi = {10.70728/a.series.tab.v08.i09.159},
  url = {https://doi.org/10.70728/a.series.tab.v08.i09.159}
}

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