The aim of this study is determination of optimal fermentation conditions of the Pichia pastoris yeast expressing the recombinant Pres2-S protein of hepatitis B virus (HBV). For this purpose we investigated the main factors influencing for the cultivation of recombinant strain of the yeast Pichia pastoris pPIC3,5-S-HBsAg - the composition of the nutrient medium, pH and dissolved oxygen (DO). As a result, by enzyme-linked immunosorbent assay (ELISA) the optimal concentration of methanol ≈1.0 %, which is the initiator and the only source of carbon for protein expression in the Mut+ phenotype Pichia pastoris yeast cells was determined. In the selected conditions at the end of the fermentation process, the wet biomass of yeast cells was 420 g/l.
| Jurnal | Kimyo va kimyo texnologiyasi |
|---|---|
| Nashr sanasi | 2020-12-04 |
| Jild | 2020 |
| Son | 3 |
| Betlar | 56-59 |
| Til | en |
| DOI | 10.51348/mpau8653 |
DOI: 10.51348/mpau8653 · Maqolaning asl sahifasi
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