The aim of this study is determination of optimal fermentation conditions of the Pichia pastoris yeast expressing the recombinant Pres2-S protein of hepatitis B virus (HBV). For this purpose we investigated the main factors influencing for the cultivation of recombinant strain of the yeast Pichia pastoris pPIC3,5-S-HBsAg - the composition of the nutrient medium, pH and dissolved oxygen (DO). As a result, by enzyme-linked immunosorbent assay (ELISA) the optimal concentration of methanol ≈1.0 %, which is the initiator and the only source of carbon for protein expression in the Mut+ phenotype Pichia pastoris yeast cells was determined. In the selected conditions at the end of the fermentation process, the wet biomass of yeast cells was 420 g/l.
| Jurnal | Kimyo va kimyo texnologiyasi |
|---|---|
| Nashriyot | Тошкент кимё-технология институти |
| ISSN | 1992-9498, 2181-1121 |
| Nashr sanasi | 2020-12-04 |
| Jild | 2020 |
| Son | 3 |
| Betlar | 56-59 |
| Til | Ingliz |
| DOI | 10.51348/mpau8653 |
| Indeksga qo‘shilgan | 2026-09-02 |
DOI: 10.51348/mpau8653 · Maqolaning asl sahifasi
| APA | Noma’lum muallif (2020). OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV). Kimyo va kimyo texnologiyasi, 2020(3), 56-59. https://doi.org/10.51348/mpau8653 |
|---|---|
| GOST (OAK) | Noma’lum muallif OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV) // Kimyo va kimyo texnologiyasi. – 2020. – T. 2020, № 3. – P. 56-59. – DOI: 10.51348/mpau8653. |
| MLA | Noma’lum muallif. “OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV).” Kimyo va kimyo texnologiyasi, 2020(3), 56-59, 2020. https://doi.org/10.51348/mpau8653 |
| Chicago | Noma’lum muallif. “OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV).” Kimyo va kimyo texnologiyasi, 2020(3), 56-59 (2020). https://doi.org/10.51348/mpau8653 |
| Harvard | Noma’lum muallif (2020) ‘OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV)’, Kimyo va kimyo texnologiyasi, 2020(3), 56-59. https://doi.org/10.51348/mpau8653 |
| BibTeX | @article{article2020,
title = {OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV)},
author = {Unknown},
journal = {Kimyo va kimyo texnologiyasi},
year = {2020},
volume = {2020},
number = {3},
pages = {56-59},
doi = {10.51348/mpau8653},
url = {https://doi.org/10.51348/mpau8653}
} |
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