This study is devoted to the optimization and validation of the DPPH method for the evaluation of antiradical activity of plant compositions. The optimal analytical parameters, including DPPH concentration, measurement wavelength, incubation time, and extract volume range, were determined. The main validation characteristics of the method — linearity, sensitivity, precision, and specificity — were assessed. The results confirm that the DPPH assay is a reliable and reproducible tool for determining the antiradical activity of plant extracts. The proposed approach can be applied for the standardization of phytocompositions and screening of natural antioxidants.
| Mualliflar | Askarov, Akmaljon N. |
|---|---|
| Jurnal | Товарлар кимёси ва халқ табобати |
| Nashr sanasi | 2026-03-21 |
| Jild | 5 |
| Son | 1 |
| Betlar | 356-386 |
| Til | O‘zbek |
| DOI | 10.55475/jcgtm/vol5.iss1.2026.604 |
DOI: 10.55475/jcgtm/vol5.iss1.2026.604 · Maqolaning asl sahifasi
DPPH assay, antioxidant activity, common tansy (Tanacetum vulgare L.), field horsetail (Equisetum arvense L.), method validation, ultrasound extraction, spectrophotometry, free radicals, flavonoids, phenolic compounds, DPPH-тест, антиоксидантная активность, пижма обыкновенная, Tanacetum vulgare L., хвощ полевой, Equisetum arvense L., валидация методики, ультразвуковая экстракция, спектрофотометрия, свободные радикалы, флавоноиды, фенольные соединения, DPPH-testi, antioksidant faollik, dastarbosh (Tanacetum vulgare L.), dala qirqbo‘g‘ini (Equisetum arvense L.), metodika validatsiyasi, ultratovushli ekstraksiya, spektrofotometriya, erkin radikallar, flavonoidlar, fenol birikmalar
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