The aim of the study is to obtain polymolecular complexes based on chitosan and Bombyx mori protein. To obtain complexes, protein and chitin are isolated from silk production waste, from which chitosan is synthesized. The optimal parameters for obtaining chitosan and protein determined: temperature, reaction time, concentration of components. The molecular weight and degree of deacetylation of chitosan determined, which are 95-140 kDa and 77-90%, respectively. The fact of the formation of protein and chitosan proved by IR spectroscopy and elemental analysis. The definition of amino acid analysis of the protein showed that 16 amino acids identified in the protein, nine of which are essential. Studies have been carried out on the antibacterial activity of the Bombyx mori protein to suppress various strains of microorganisms under in vitro conditions. We found that the Bombyx mori protein has a bactericidal effect on both gram-negative and gram-positive bacteria. Polymolecular protein-chitosan complexes obtained by potentiometric titration of protein solutions with chitosan. A technological scheme developed for obtaining complexes . The obtained complexes tested at the Research Institute for the Development of Fish Farming as part of compound feed. A preparation based on protein and chitosan Bombyx mori leads to increase of the fish survival, an increase of the growth rate of fish and a decrease of the feed coefficient.
| Mualliflar | Rakiya Yu. Milusheva, С. Ш. Рашидова |
|---|---|
| Jurnal | Kimyo va kimyo texnologiyasi |
| Nashr sanasi | 2024-03-28 |
| Jild | 2023 |
| Son | 1 |
| Til | en |
| DOI | 10.70189/1992-9498.1583 |
DOI: 10.70189/1992-9498.1583 · Maqolaning asl sahifasi
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