Plastic pollution, primarily associated with polyethylene terephthalate (PET) plastics, has become a major global environmental crisis. Ideonella sakaiensis strain 201-F6 is the most effective solution to degrade PET. In this article, we described the results of studies on cloning the FAST-PETase enzyme with thermostable mutations and its expression in E.coli bacteria, purifying the protein by nickel chelate chromatography, and determining the enzyme activity. The study results showed that the active FAST-PETase enzyme was effectively purified.
| Mualliflar | Muhammad Latif NAZIROV, Ilxom XALILOV,, Fazliddin QOBILOV, |
|---|---|
| Jurnal | Ўзбекистон Миллий университети хабарлари |
| Nashr sanasi | 2024-03-30 |
| Jild | 3 |
| Son | 3.1 |
| Betlar | 81-85 |
| Til | O‘zbek |
| DOI | 10.69617/nuuz.v3i3.1.1718 |
DOI: 10.69617/nuuz.v3i3.1.1718 · Maqolaning asl sahifasi
деградация пластика ПЭТ, Ideonella sakaiensis, ПЭТаза, ферментная инженерия, сигнальный пептид., PET plastic degradation, Ideonella sakaiensis, PETase, enzyme engineering, signal peptide., PET plastik degradatsiyasi, Ideonella sakaiensis, PETaza, ferment muhandisligi, signal peptid.
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