This article describes the indicators of DNA content in the nuclei of cell suspensions during flow cytophotometry, which differ significantly from the results of photometry of histological and cytological preparations stained according to Feulgen. With flow photometry, the amount of DNA is measured not only in the nuclei of epithelial cells in neoplasia, but also in the nuclei of cells in the stroma, blood vessels, and other structures. These conditions of flow photometry significantly change the nature of the histograms of the distribution of nuclei, as a rule, towards the predominance of the diploid peak due to the nuclei of non-tumor cells. If monoclonal antibody "tags" are used in flow cytometry to detect the cell population of interest, then in this case the result of the study may also be unreliable, since monoclonal antibodies can be expressed both in carcinomas and in benign tumors and normal breast tissue. When diagnosing the last stage, the degree of differentiation of carcinoma is determined by ploidy values. The ploidy of cell nuclei 4.5-5.4s indicates the first, highly differentiated degree, 5.5-6.4s the second, moderately differentiated degree, 6.5-7.4s - the third, low-differentiated. Thus, this research method makes it possible to clarify the diagnosis of the stages of carcinogenesis in the mammary gland and make informed decisions regarding benign processes, borderline conditions, and malignant neoplasms. The data obtained can be used as additional differential diagnostic criteria for morphological diagnosis of the stages of carcinogenesis in the mammary gland.
| Mualliflar | ALIMKHODZHAEVA Lola Telmanovna, BOZOROVA Lutfiya Makhmudovna |
|---|---|
| Jurnal | Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali |
| Nashr sanasi | 2023-02-07 |
| Jild | 7 |
| Son | 6 |
| Til | Rus |
Рак молочной железы, канцерогенез, диагностика, факторы прогноза, Breast cancer, carcinogenesis, diagnosis, prognostic factors, Sut bezi saratoni, kanserogenez, diagnostika, prognostik omillar
One of the most urgent problems today is the problem of early diagnosis of non-invasive ductal carcinoma. Diagnostic and therapeutic tactics in NIPK is largely uncertain, since in many cases the tumor is characterized…
One of the most pressing issues in contemporary pediatrics and nephrology is chronic kidney disease (CKD), which is linked to early handicaps in patients as early as childhood, the emergence of chronic renal failure…
Objective: to improve the results of surgical treatment of oral cancer using a pectoral flap. Methods: Under our observation there were 35 patients with oral cancer who underwent reconstructive plastic surgery using a…
Identifying the underlying cause of muscle hypotonia remains difficult, despite advances in diagnostic laboratories and imaging techniques. Clinical assessment strategies and standardised developmental tests can help to…
Objective: to study the results of diagnosis and targeted therapy of patients with colorectal cancer (CRC) with liver metastases. Methods: The study included 75 patients with metastatic CRC, including 39 (52.0%) men and…
The Decree of the President of the Republic of Uzbekistan dated February 26, 2019, “On measures to strengthen further the social protection of employees of internal affairs bodies and members of their families” PD-4212…
Objective: to study clinical aspects and tactics of treatment of patients with orbital wall lesions with regard to visual function Methods: analysis of a comprehensive clinical examination of 62 patients with…
The article discusses the treatment with the use of angiogenesis inhibitors in the complex treatment of diabetic macular edema. A comparative analysis of all types of anti-VGEF drugs for macular edema is given. The use…
Accurate data on the epidemiology of biliary obstruction have not been obtained. Obstructive cholestases in children of early age correspond to BA in 68-75% of points, and in 12-15% of points to cystic transformation of…
Objective: to study the prevalence of recurrent obstructive bronchitis in the structure of childhood morbidity. Methods: in the course of the work, a retrospective analysis of 13246 medical histories of children who…
Journal of Biomedicine and Practice – Biomeditsina va amaliyot jurnali — barcha maqolalar