Based on pPIC3.5 and pPIC9 yeast vectors the recombinant plasmid DNA pPIC3.5-GFP (8464 bp) and pPIC9-GFP (8718 bp) containing cDNA (719 bp) of Green fluorescent protein (GFP) were cloned. The obtained plasmids were transformed into yeast cells of the GS115 Pichia pastoris strain. Transformants are selected on histidine-deficient medium. Recombinant clones of pPIC3.5-GFP and pPIC9-GFP were identified by detecting cell fluorescence. GFP expression in yeast is confirmed by green fluorescence. Using electrophoresis in PAAG the molecular weight of the synthesized protein (≈27 kDa) is determined. The resulting reporter constructs and recombinant strains can be used to study the expression level of various recombinant proteins in Pichia pastoris cells.
| Mualliflar | Ashirоv Оybek, Sasmakоv Sobirdjan, Abdurakhmanоv Jaloliddin, HASANОV Shuhrat |
|---|---|
| Jurnal | Kimyo va kimyo texnologiyasi |
| Nashr sanasi | 2020-01-12 |
| Jild | 2019 |
| Son | 4 |
| Til | en |
| DOI | 10.70189/1992-9498.1136 |
DOI: 10.70189/1992-9498.1136 · Maqolaning asl sahifasi
The influence of the nature of aldehydes of the structure of the products of their interaction with diethanolamine was studied. It was found that the interaction of one mole of diethanolamine with one mole of aldehyde…
RPF-fuel granules consisted of paper and plastic wastes in various ratios, and activated carbons based on RPF were obtained. The optimal conditions of steam activation in the nitrogen atmosphere are revealed. Physically…
The aim of this work was to obtain corrosion inhibitors. It has been established that heterocyclic compounds, including pyridine and its derivatives, are more frequently used as the active component in the combination…
Results of researches and calculations summarized for improving the technology of flotation potassium chloride from sylvinites of the Tyubegatan deposit. The possibility of decrease production costs associated with the…
The description of a pilot plant and results of a research of intensity of a heat mass transfer are provided and hy-draulic resistance at various initial concentration of the absorbed gas and relations of an expense of…
In this Articie the process of influence of modified bentonite on the interphase interaction of rubbers was invistigated. The influence of bentonite particles and the content of the modifier on the technological and…
The aim of the study was to study the conditions of copolycondensation of phenol alcohol with hydroxyl-containing polyether polyol in the presence of maleic anhydride chain extender in order to obtain modified thermoset…
In the inner mixing zone of the tested suspended extractor, the fluid and gas flows in the stream and the external mixing zone moves in a contradictory flow. In order for these mixing zones to operate equally in…
The sorption and complex-forming properties of PAD600-based impregnated sorbents with respect to the Pt (II) ion were studied. The parameters of sorption extraction of platinum ions are determined. It was found that…
The aim of the study is to determine the optimal composition of the composition of synthetic detergents having a high functional property from local raw materials. For the preparation of synthetic detergents as a source…